Expression and regulation of two RFamide peptide genes in the rat
نویسندگان
چکیده
6 List of original publications 7 List of abbreviations 8 1. Review of the literature 9 1.1 General aspects of neuropeptides 9 1.2 The RFamide family of neuropeptides 11 1.3 Biosynthesis and secretion of neuropeptides 13 1.4 Neuropeptide receptors 15 1.5 Functional properties of neuropeptides 17 1.5.1 Energy and fluid homeostasis 17 1.5.2 Neuropeptides and prolactin secretion 19 1.5.3 Cardiovascular control 19 1.5.4 Neuropeptides, adrenal hormones and the immune system 21 1.5.5 Neuropeptides and ingestion 22 1.6 Role of neuropeptides in the development 23 1.7 Mechanisms of pain and involvement of neuropeptides 24 1.7.1 Pain circuits 25 1.7.2 Pain modulatory pathways 26 1.7.3 Pain and RFamides 27 2. Aims of the study 29 3. Materials and methods 30 3.1 Ethics 30 3.2 Animals 30 3.3 Experimental models 30 3.3.1 Hyperosmolar stimulus 30 3.3.2 Methods for assessing pain 31 3.3.3 Injections 31 3.4 Cloning 31 3.5 Immunohistochemistry 32 3.6 In situ hybridisation 32 3.7 Measurements and statistics 33 3.7.1 Measurements 33 3.7.2 Quantification of gene expression 33
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P-222: Expression of RFamide-Related Peptide-3 and KiSS-1 mRNA in The Hypothalamus of Pregnant Rat
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